The anti-NKG2D chimeric antigen receptor (CAR) Jurkat cell line is a stable cell line derived from anti-NKG2D CAR lentivirus transduction. This transduced CAR lentiviral vector was constructed to express scFv of an anti-NKG2D antibody linked to the CD28 and CD3ζ signaling domains. And this recombinant cell product can be used to target human NKG2D and treat Colorectal cancer.