Summary: Total GPCR Internalization cells are engineered to co-the ProLink (PK) tagged GPCR, and an Enzyme Acceptor (EA) tag localized to the endosomes. Activation of the GPCR-PK induces internalization of the receptor in EA-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
Agriculture & Food
Business Services
Electronics, IT and Telecoms
Leisure & Tourism
Minerals
Textiles, Clothing, Leather,
Transport & Logistics
