The Ultimate Guide to Fluorescent Dye 
Fluorescent dyes, also known as fluorophores, are compounds that absorb light at one wavelength and emit light at a higher wavelength, resulting in fluorescence. These fluorescent dyes can be categorized into various groups, including organic dyes such as fluorescein, rhodamine, and AMCA, biological fluorophores like green fluorescent protein, phycoerythrin, and allophycocyanin, and quantum dots. They are commonly employed in labeling biomolecules with fluorescence. Notably, they offer advantages of smaller size and greater photostability compared to fluorescent proteins.

Furthermore, fluorophores are considered safe for usage and do not necessitate radiological controls. Multiple fluorophores can be utilized simultaneously without interference as long as their emission spectra do not overlap.

Fluorescent Dyes Examples by Emission Color
Blue/Cyan Emitting Fluorescent Dyes:
Hoechst dyes: Emit blue fluorescence and are commonly used for DNA staini