Summary: Cat.No. : GPT-59H Product Overview : Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Description : GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Source : E. coli Species : Human Tag : His Form : Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT Bio-activity : Specific activity is >100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C Molecular Mass : 56.8 kDa (516aa), confirmed by MALDI-TOF