Summary: In this system, the interaction occurs at the very tip of a glass fiber-based BLI biosensor. White light travels down the glass fiber and is reflected back up from two interfaces at the tip: internal interface and external interface. When molecules bind to the surface of the biosensor, the thickness variation of the external layer of immobilized protein causes a shift of the interference pattern. The pattern can be plotted as a nanometer shift of relative intensity vs. wavelength that can be recorded in real-time with high accuracy.
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