Summary: At profacgen, our scientists have developed a high-throughput workflow for examining such interactions with an emphasis on identifying the binding site of a target protein. Based on the sequence of the target protein, overlapping peptides are synthesized as 15 or 20 mers which covers the full sequence of the protein. The peptide library is immobilized on the surface of a sensor chip and the analyte molecule (the interacting protein) is injected in the solution through the flow cell. By monitoring the change in the intensity of the reflected light (caused by the change in refractive index), peptides are screened against the analyte protein and those that can associate with the analyte are identified, which correspond to the regions of the target protein where binding occurs.
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