Summary: The resonance also requires that the frequency of incident photon matches the natural oscillating frequency of the surface plasmon. Once the resonance happens, the energy of the reflected photon will be absorbed and the light dramatically weakened. Within the SPR angle range, the SPR angle (or resonance angle) is defined as the angle at which nearly no reflect photon can be detected. Since the introduction of SPR into the immune sensor field by Liedberg, Nylander and Lundström in 1983, surface plasmon optical biosensor has been extensively studied and widely used. Later on, the SPR technology was further developed to study the interaction between ligand and analyte on a biosensor chip. As the SPR angle changes with surface refractive indexes, which is in direct proportion to the molecular mass of the biomolecule attached to the metal surface, the binding of the analyte can be detected at high sensitivity.
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