Summary: With years of experience, Profacgen has accomplished to significantly increase the expression level and optimize the purification scheme of recombinant trimeric fusion proteins, the scale can be quickly moved from preclinical stage towards the bedsides of millions of patients. Our protein trimerization technology has established a broadly applicable system for the production and characterization of trimeric proteins and generated new insights into the assembly and maturation of trimers that will have implications for the design of an effective vaccine.