cAMP Direct Immunoassay Detection Kit (Fluorometric)
TypeName: Business Offer
Category:
Offer Date:2019/07/03
Country: United States
Summary: cAMP Direct Immunoassay Detection Kit (Fluorometric) provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay systems. Compared to other ELISA cAMP assay kits, this kit eliminates the tedious acetylation step. The kit uses AbRed Indicator as a fluorimetric substrate to quantify the HRP activity. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate. This kit is based on the competition between HRP-labeled cAMP and free cAMP for a fixed number of cAMP antibody binding sites. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test samples competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits th
Description: Adenosine 3", 5" cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of GPCRs. http://www.creativebiomart.net/cAMP-Direct-Immunoassay-Detection-Kit-Fluorometric-462455.htm
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