Summary: This product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR, FDSS, BMG NOVOstar, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.If you would like to quantify calcium concentration in vitro using cell extracts, we recommend using Calcium Detection Kit (Colorimetric) or Calcium Quantification Assay Kit, as they provide stock standards for comparison.