Summary: Sensitive assays of maleimide and thiol groups are required for the efficient conjugation of proteins that are expensive and available only in small amounts. A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules. There are few reagents or assay kits available for quantifying the number of maleimide groups that are introduced into the first protein. All the commercial kits have tedious protocols.
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