Summary: Cell-Free Protein Expression Kit is designed for in vitro transcription and translation of target DNA to protein in a single reaction. This product contains an optimized E.coli extract containing T7 RNA polymerase for transcription and ribosome for translation. An optimized Master mix provides all other required components, including amino acids, rNTPs, and appropriate salts to express high-level production of recombinant proteins. This product contains reagents for expression of protein directly from a variety of DNA templates, includingT7 promoter and terminator as well as RBS (ribosomal binding site). This product can be used to express a wide range of target proteins and has the capacity for a yield of more than 300 µg/mL of protein within 3 hours
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