Summary: Glutamic Oxaloacetic Transaminase Activity Assay Kit provides a quick and sensitive method for the measurement of GOT (glutamic oxaloacetic transaminase) in various biological samples. GOT catalyzes the reaction of aspartate and α-ketoglutarate to oxaloacetate and glutamate:Aspartate (Asp) + α-ketoglutarate ? oxaloacetate + glutamate (Glu)The product L-glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle. The signal can be read by a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm). It can detect as little as 2 mU/mL AST in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.
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