Summary: Neuraminidase Assay Kit (Fluorometric-Blue) provides a sensitive and robust fluorometric assay to detect neuraminidase that exists either in cells or biological samples. The non-fluorescent neuraminidase substrate becomes strongly fluorescent upon neuraminidase cleavage. The kit can detect as little as 0.3 mU/mL neuraminidase in a 100 µL assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The signal can be easily read by a fluorescence microplate reader at Ex/Em = ~320/~450 nm.
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