Summary: Easy-to-use kits (two-step) for screening SIRT5 inhibitors/activators; includes enough active enzyme for entire plateIncludes optimal substrate selected from a panel of succinylated sites96-well plate included, but can be adapted to higher well formatControl inhibitors includedSuitable for high-throughput screening (Z"-factors >0.73)Optimal, specific SIRT5 substrate means low enzyme concentration, making "hit" validation easyWhile the FLUOR DE LYS-Succinyl substrate contains an AMC ("blue") fluorophore that uses commonly-used wavelengths, FLUOR DE LYS-Succinyl Green substrate has longer excitation/emission wavelengths, avoiding interference often seen with screening compounds at shorter wavelengths.
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