Summary: Easy-to-use kits (two-step) for screening SIRT5 inhibitors/activators; includes enough active enzyme for entire plateIncludes optimal substrate selected from a panel of succinylated sites96-well plate included, but can be adapted to higher well formatControl inhibitors includedSuitable for high-throughput screening (Z"-factors >0.73)Optimal, specific SIRT5 substrate means low enzyme concentration, making "hit" validation easyThe SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats, including cuvettes and 384-well plates. The SIRT5 Fluorometric Activity Assay is based on the unique FLUOR DE LYS-Succinyl Substrate/Developer combination. The assay procedure has two steps. The FLUOR DE LYS-Succinyl Substrat
Agriculture & Food
Business Services
Electronics, IT and Telecoms
Leisure & Tourism
Minerals
Textiles, Clothing, Leather,
Transport & Logistics
