Description: Sphingomyelinase Assay Kit (Colorimetric) provides a sensitive method for detecting neutral SMase activity or screening its inhibitors. The kit uses our proprietary AbBlue Indicator as a colorimetric probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). It can be used for measuring the SMase activity in blood, cell extracts or other solutions. The absorbance of light at 655 nm is proportional to the formation of phosphocholine, therefore to the SMase activity. The kit is an optimized "mix and read" assay that is compatible with HTS liquid handling instruments.
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