Summary: Superoxide dismutase (SOD) are metalloenzymes that catalyze the dismutation of superoxide radical into hydrogen peroxide (H2O2) + molecular oxygen (O2) and consequently provide an important defense mechanism against superoxide radical toxicity. SOD has shown to protect murine peritoneal macrophages from apoptosis induced by adriamycine. Additionally, the over expression of SOD in fibrosarcome cells, has exhibited protection against apoptosis and promotes cell differentiation. This Superoxide Dismutase Detection Kit utilizes a water-soluble tetrazolium salt, WST-1 (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) that produces a highly water-soluble formazan dye upon reduction with a superoxide anion.
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