Summary: PTP1B Inhibitor Screening Assay Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity. The kit includes Human, recombinant PTP1B (residues 1-322; MW=37. 4 kDa), expressed in E. coli as a positive control. The PTP1B inhibitor suramin is including as control for inhibitor detection. The detection of free-phosphate released is based on the classic Malachite green assay and offers the advantages of convenient, one-step detection and excellent sensitivity, without radioactivity.
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