Gene Knockout Stable Cell Lines

Pharmaceuticals

Gene Knockout Stable Cell Lines

Creative Biogene

Our scientists have years of experience at performing gene editing with CRISPR/Cas9, from designing gRNA to transfection and single clone generation. Creative Biogene is offering a series of gene knockout cell lines developed by CRISPR/Cas9 system. These cell lines provide you with a convenient means to study gene functions.

The CRISPR/Cas9 system is an adaptive immunity system in bacteria against bacteriophage invading. It has been developed as the most popular method for genome editing in recent years. The engineered CRISPR/Cas9 technology consists of a single guide RNA (sgRNA) and a human codon-optimized Cas9 nuclease. The sgRNA recognizes the genomic target via standard Watson–crick base pairing and acts as a scaffold for binding of the endonuclease Cas9. Then Cas9 protein introduces site-specific DSBs in the target DNA sequences. Besides, in the phase of target recognition, the protospacer-adjacent motif (PAM) adjacent to the 3' end of the DNA target site is indispensable. Finally, targeted mutations can be induced by nonhomologous end joining (NHEJ).

Gene Knockout Stable Cell Lines